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adamts13 cleaved vwf  (R&D Systems)


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    R&D Systems adamts13 cleaved vwf
    Adamts13 Cleaved Vwf, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/adamts13+cleaved+vwf/Human+vWF-A2+(ADAMTS13-cleaved)+Antibody/pmc12481898-78-0-12
    Average 93 stars, based on 6 article reviews
    adamts13 cleaved vwf - by Bioz Stars, 2026-11
    93/100 stars

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    Enzyme-linked Immunosorbent Assay:

    Article Title: Plasmin-mediated proteolysis of von Willebrand factor in patients with acute and chronic liver disease
    Article Snippet: ADAMTS13 antigen levels were quantified using a commercially available ELISA kit (R&D Systems). .. ADAMTS13-cleaved VWF was measured by ELISA using a neoepitope-specific monoclonal antibody (MAB27642; R&D Systems), according to the method described by Rauch et al. [ ]. cVWF levels were measured by ELISA using variable domain of heavy-chain-only antibodies as previously described by El Otmani et al. [ ]. .. ADAMTS13 antigen levels were quantified using a commercially available ELISA kit (R&D Systems).



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    Assay principle. (A) The plasma sample is diluted 1 in 10 in substrate solution (5 mM BaCl 2 ) containing 316 nM biotinylated (●-) vWF-substrate (vWF73) and incubated for 30 min at 37 °C under shaking. (B) After stopping of the reaction with EDTA, samples are diluted and Alexa Fluor 647-labeled, and streptavidin-coated beads as well as a PE-labeled mAb (Y○) that binds to the <t>ADAMTS-13</t> cleavage site (PE–mAb) are added. (C) After incubation, samples are further diluted and Alexa Fluor-labeled beads gated by FSC/SSC (left panel) or fluorescence (Alexa Fluor 647, FL6, right panel) emission characteristics. (D) Corresponding MFI values (R-PE, FL2) represent the ADAMTS-13 activity in the original plasma sample (data of plasma calibrators shown).
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    Assay principle. (A) The plasma sample is diluted 1 in 10 in substrate solution (5 mM BaCl 2 ) containing 316 nM biotinylated (●-) vWF-substrate (vWF73) and incubated for 30 min at 37 °C under shaking. (B) After stopping of the reaction with EDTA, samples are diluted and Alexa Fluor 647-labeled, and streptavidin-coated beads as well as a PE-labeled mAb (Y○) that binds to the ADAMTS-13 cleavage site (PE–mAb) are added. (C) After incubation, samples are further diluted and Alexa Fluor-labeled beads gated by FSC/SSC (left panel) or fluorescence (Alexa Fluor 647, FL6, right panel) emission characteristics. (D) Corresponding MFI values (R-PE, FL2) represent the ADAMTS-13 activity in the original plasma sample (data of plasma calibrators shown).

    Journal: ACS Omega

    Article Title: Assay for ADAMTS-13 Activity with Flow Cytometric Readout

    doi: 10.1021/acsomega.2c02077

    Figure Lengend Snippet: Assay principle. (A) The plasma sample is diluted 1 in 10 in substrate solution (5 mM BaCl 2 ) containing 316 nM biotinylated (●-) vWF-substrate (vWF73) and incubated for 30 min at 37 °C under shaking. (B) After stopping of the reaction with EDTA, samples are diluted and Alexa Fluor 647-labeled, and streptavidin-coated beads as well as a PE-labeled mAb (Y○) that binds to the ADAMTS-13 cleavage site (PE–mAb) are added. (C) After incubation, samples are further diluted and Alexa Fluor-labeled beads gated by FSC/SSC (left panel) or fluorescence (Alexa Fluor 647, FL6, right panel) emission characteristics. (D) Corresponding MFI values (R-PE, FL2) represent the ADAMTS-13 activity in the original plasma sample (data of plasma calibrators shown).

    Article Snippet: The anti-human vWF-A2 (ADAMTS-13 cleaved)-specific mAb (clone #490628) was ordered from Bio-Techne GmbH (Wiesbaden-Nordenstadt, Germany).

    Techniques: Incubation, Labeling, Fluorescence, Activity Assay

    General assay performance. A total of nine different plasma ADAMTS-13 calibrators covering a range from 0 to 0.91 IU/mL were prepared by diluting the WHO international standard for plasma ADAMTS-13 activity in heat-inactivated PNP. All calibrators were introduced to three independent experiments, and the corresponding results are shown as MFIs ± SD that reflect binding of the R-PE-labeled mAb used for assay readout. The small figure shows the clinically relevant lower concentration range (0 to 0.2 IU/mL).

    Journal: ACS Omega

    Article Title: Assay for ADAMTS-13 Activity with Flow Cytometric Readout

    doi: 10.1021/acsomega.2c02077

    Figure Lengend Snippet: General assay performance. A total of nine different plasma ADAMTS-13 calibrators covering a range from 0 to 0.91 IU/mL were prepared by diluting the WHO international standard for plasma ADAMTS-13 activity in heat-inactivated PNP. All calibrators were introduced to three independent experiments, and the corresponding results are shown as MFIs ± SD that reflect binding of the R-PE-labeled mAb used for assay readout. The small figure shows the clinically relevant lower concentration range (0 to 0.2 IU/mL).

    Article Snippet: The anti-human vWF-A2 (ADAMTS-13 cleaved)-specific mAb (clone #490628) was ordered from Bio-Techne GmbH (Wiesbaden-Nordenstadt, Germany).

    Techniques: Activity Assay, Binding Assay, Labeling, Concentration Assay

    Comparative analysis of patient plasma samples. Samples were analyzed by a commercial ADAMTS-13 activity ELISA used for routine analysis and the flow cytometric assay described in the present paper. The gray box represents the range below the clinical threshold for TTP (<0.1 IU/mL). The triangle represents the sample used for further analysis of assay precision at low plasma ADAMTS-13 activity.

    Journal: ACS Omega

    Article Title: Assay for ADAMTS-13 Activity with Flow Cytometric Readout

    doi: 10.1021/acsomega.2c02077

    Figure Lengend Snippet: Comparative analysis of patient plasma samples. Samples were analyzed by a commercial ADAMTS-13 activity ELISA used for routine analysis and the flow cytometric assay described in the present paper. The gray box represents the range below the clinical threshold for TTP (<0.1 IU/mL). The triangle represents the sample used for further analysis of assay precision at low plasma ADAMTS-13 activity.

    Article Snippet: The anti-human vWF-A2 (ADAMTS-13 cleaved)-specific mAb (clone #490628) was ordered from Bio-Techne GmbH (Wiesbaden-Nordenstadt, Germany).

    Techniques: Activity Assay, Enzyme-linked Immunosorbent Assay, Flow Cytometry